Download e-book for kindle: A Practical Guide to Basic Laboratory Andrology by Lars Bjorndahl, David Mortimer, Christopher L. R. Barratt,

By Lars Bjorndahl, David Mortimer, Christopher L. R. Barratt, Jose Antonio Castilla, Roelof Menkveld, Ulrik Kvist, Juan G. Alvarez, Trine B. Haugen

ISBN-10: 0521735904

ISBN-13: 9780521735902

This useful, largely illustrated guide covers the systems which are undertaken in andrology and artwork laboratories to examine and investigate male-factor infertility, and to arrange spermatozoa to be used in assisted belief remedy. The content material is gifted as short, authoritative overviews of the proper organic history for every region, plus specified, step by step descriptions of the suitable analytical methods. every one technical part comprises pertinent quality controls issues, in addition to the optimal presentation of effects. as well as the great 'basic' semen research, incorporating cautious research of sperm morphology, the guide offers verified recommendations for using computer-aided sperm research and sperm sensible evaluation. through the instruction manual the translation of laboratory leads to the scientific context is highlighted, and secure laboratory perform is emphasised. it truly is a useful source to all scientists and technicians who practice diagnostic checking out for male-factor infertility.

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High semen volume has also been associated with a high risk for semen loss from the vagina. Causes for increased semen volume can be: * Inflammatory exudate from, for instance, an infected prostate during the acute (exudative) phase of inflammation * Extensive sexual stimulation, possibly combined with long abstinence time Semen liquefaction Principles The ejaculate coagulates due to macro-proteins originating from the seminal vesicles. In the laboratory the entire ejaculate is brought together in one collection container, including the 37 Chapter 3: Basic semen analysis first sperm-rich fraction, and all fractions are involved in the coagulum.

Gynecol Invest 1975; 6: 206–14. 29. Mortimer D, Templeton AA. Sperm transport in the human female reproductive tract in relation to semen analysis characteristics and time of ovulation. J Reprod Fertil 1982; 64: 401–8. 30. Suarez SS, Pacey AA. Sperm transport in the female reproductive tract. Hum Reprod Update 2006; 12: 23–37. 31. Mortimer D. Sperm transport in the female genital tract. In: Grudzinskas JG, Yovish JL, eds. Gametes – The Spermatozoa. Cambridge: Cambridge University Press 1995. 32.

A change in semen osmolarity in vitro affects sperm selection in vitro In vivo, spermatozoa are expelled in the isotonic prostatic fluid onto the isotonic cervical mucus extruding from the cervical opening into the vagina. In vitro, enzymatic digestion 21 Chapter 2: Basic physiology of macromolecules starts at ejaculation, resulting in a rapid increase of the semen osmolarity from 290 up to 450 mosmol/l three hours later [10]. There are two implications of this. One is the possible direct effect of the increasing osmolarity on spermatozoa, while the other is the risk and effects of hypo-osmotic stress, when spermatozoa in hypertonic seminal plasma are exposed to isotonic culture media.

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A Practical Guide to Basic Laboratory Andrology by Lars Bjorndahl, David Mortimer, Christopher L. R. Barratt, Jose Antonio Castilla, Roelof Menkveld, Ulrik Kvist, Juan G. Alvarez, Trine B. Haugen


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